Description: Serum, Plasma

Description: True Blue

Description: True Blue

Description: True Blue

Description: The GLS2 Inhibitor Screening Assay Kit is designed to measure the hydrolase activity of GLS2 for screening and profiling applications.

Description: UBE1 dysregulation has been linked to muscular atrophy, tumors, and cancer. The UBE1 Inhibitor Screening Assay Kit is designed to measure UBE1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. 

Description: The ARG2 Inhibitor Screening Assay Kit is designed to measure ARG2 enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions. The ARG2 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing thioarginine substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=410-415 nm.

Description: The ARG2 Inhibitor Screening Assay Kit is designed to measure ARG2 enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions. The ARG2 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing thioarginine substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=410-415 nm.

Description: The ARG1 Inhibitor Screening Assay Kit is designed to measure ARG1 enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions. The ARG1 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing thioarginine substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=410-415 nm.

Description: The ARG1 Inhibitor Screening Assay Kit is designed to measure ARG1 enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions. The ARG1 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing thioarginine substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=410-415 nm.

Description: The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

Description: The TPH2 Assay Kit is designed to measure TPH2 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple -
just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

Description: The CD73 Inhibitor Screening Assay Kit is designed to measure CD73 activity_x000D_for screening and profiling applications. The CD73 assay kit comes in a convenient 96-well_x000D_format, with purified recombinant CD73 enzyme, AMP, CD73 assay buffer, and Colorimetric_x000D_detection reagent for 100 enzyme reactions.

Description: The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 384 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

Description: The TPH2 Assay Kit is designed to measure TPH2 enzyme inhibition in a 384 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple -
just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

Description: The CD73 Inhibitor Screening Assay Kit is designed to measure CD73 activity_x000D_for screening and profiling applications. The CD73 assay kit comes in a convenient 384-well_x000D_format, with purified recombinant CD73 enzyme, AMP, CD73 assay buffer, and Colorimetric_x000D_detection reagent for 384 enzyme reactions.

Description: The USP1 Inhibitor Screening Assay Kit is a 96-well format fluorogenic assay kit designed to measure the activity of the deubiquitinating (DUB) enzyme USP1 (ubiquitin specific peptidase 1) for screening and profiling applications. The kit contains enough purified USP1/UAF1 (ubiquitin specific peptidase 1 associated factor 1) complex, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP1 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of the DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteasesFigure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases, with a C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form, the energy emitted from fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with an λexcitation/λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP1? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

Description: The USP5 Inhibitor Screening Assay Kit is a 96-well format fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP5 for screening and profiling applications. The kit contains enough purified USP5 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP5 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of the DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form, the energy emitted from the fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with an λexcitation/λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP5? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

Description: The Ubiquitin specific peptidase 2 (USP2) Inhibitor Screening Assay Kit is a homogeneous fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP2 for screening and profiling applications. The kit contains enough purified USP2 recombinant protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP2 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form, the energy emitted from fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with an λexcitation/ λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP2? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

Description: The USP8 Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP8 (ubiquitin specific peptidase 8) for screening and profiling applications. The kit comes in a convenient 96-well format and contains enough purified recombinant human USP8 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP8 activity the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC (Ub-AMC) is a fluorogenic substrate for ubiquitin hydrolases, based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Upon release from Ub, AMC emissions are no longer quenched fluorescence with λexcitation/λemission maxima of 350/460 nm is emitted. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP8? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

Description: The COX1 Inhibitor Screening Assay Kit is designed to measure COX1 (cyclooxygenase 1) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant COX1, Amplex™ Red, Arachidonic Diluent, COX Assay Buffer, and 100% ethanol for 100 enzyme reactions.