Description: The human IDO1 Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human IDO1 in cultured cells.

Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4B has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP. Inhibition of PDE4B activity by its inhibitors leads to an elevated intracellular level of cAMP. The PDE4B gene encodes at least 4 different isoforms, and has been linked to inflammation in monocytes by its regulation of the toll-receptor pathway. It is also highly expressed in the central nervous system, and has been targeted as a potential treatment for autism, depression, schizophrenia, and other conditions._x000D_The PDE4B cell-based activity assay kit is designed for screening inhibitors of PDE4B1 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4B1 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4B inhibitor to inhibit PDE4B1 activity, cAMP level is restored, resulting in higher luciferase activity._x000D_The kit includes CRE luciferase reporter (premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency), PDE4B1 expression vector, and forskolin.

Description: This liquid Assay Medium is optimized for use with the PD-1 cell-based assay using the BPS Bioscience's PD-1/NFAT Reporter-Jurkat cell line (Cat #60535). _x000D_

Description: The PDE4D cell-based activity assay is designed for screening inhibitors of PDE4D7 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. _x000D_CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity.

Description: JBS True Blue

Description: Human Anti-E. coli IgG Antibody Assay Kit

Description: Human Anti-P. gingivalis IgG Antibody Assay Kit

Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
• Sensitive - highly sensitive detection of firefly luciferase activity.
• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
• Convenient - simple one-step, homogeneous protocol.
• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
• Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
• Instrumentation - does not require a luminometer with injectors.

Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
• Sensitive - highly sensitive detection of firefly luciferase activity.
• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
• Convenient - simple one-step, homogeneous protocol.
• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
• Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
• Instrumentation - does not require a luminometer with injectors.

Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
• Sensitive - highly sensitive detection of firefly luciferase activity.
• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
• Convenient - simple one-step, homogeneous protocol.
• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
• Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
• Instrumentation - does not require a luminometer with injectors.

Description: Minimum order quantity: 1 unit of 150TST

Description: Minimum order quantity: 1 unit of 1000TST

Description: The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features:• Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity.• Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time• High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications• Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.

Description: The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features:• Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity.• Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time• High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications• Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.